Publications
A microreactor for the study of biotransformations by a cross-linked γ-lactamase enzyme. Hickey, AM., Ngamsom, B., Wiles, C., Greenway, GM., Watts, P. & Littlechild, JA., (2009). Biotechnol. J., 4. In Press.
Vanadium containing bromoperoxidase - Insights into the enzymatic mechanism using X-ray crystallography. Littlechild JA., Garcia Rodriguez E. & Isupov MN., (2009). J. Inorg. Biochem. In Press
Biochemical and structural studies of a L-haloacid dehalogenase from the thermophilic archaeon Sulfolobus tokodaii.
Rye CA., Isupov MN., Lebedev AA. & Littlechild JA., (2009). Extremophiles 13, 179-90.
The binding of haem and zinc in the 1.9 A X-ray structure of Escherichia coli bacterioferritin.
Willies SC., Isupov MN., Garman EF. & Littlechild JA., (2009). J. Biol. Inorg. Chem. 14, 201-207.
The Fasciola hepatica thioredoxin: High resolution structure reveals two oxidation states
Line K., Isupov MN., Garcia-Rodriguez E., Maggioli G., Parra F. & Littlechild JA., (2008). Mol. Biochem. Parasitol. 161, 44-48.
Natural methods of protein stabilization: thermostable biocatalysts.
Littlechild JA, Guy J, Connelly S, Mallett L, Waddell S, Rye CA, Line K, & Isupov M. (2007) Biochem Soc Trans. 35:1558-63.
An order-disorder twin crystal of L-2-haloacid dehalogenase from Sulfolobus tokodaii Carrie A. Rye, Michail N. Isupov, Andrey A. Lebedev & Jennifer A. Littlechild (2007)
Acta Crystallogr D Biol Crystallogr. 63:926-30.
Tps1 regulates the pentose phosphate pathway, nitrogen metabolism and fungal virulence.
R.A.Wilson, J.M.Jenkinson, R.P Gibson, J.A. Littlechild, Z-Y. Wang & N.J.Talbot (2007) EMBO J. 26, 3673-3685.
The dodecameric vanadium dependent haloperoxidase from the marine algae Corallina officinalis: Cloning, expression and refolding of the recombinant enzyme
E. E. Coupe, M. G. Smyth, A. Fosberry, R. M. Hall & J. A. Littlechild (2007) Protein Expression and Purification, 52,265-272.
Structural studies on novel thermophilic biocatalysts
J.A.Littlechild, S.Connelly & J.Guy (2007) ISBM978-4-901833-01-1 published on line.
Crystallisation and preliminary X-ray diffraction analysis of ω-amino acid:pyruvate transaminase from Chromobacterium violaceum
Christopher Sayer, Michail Isupov & Jennifer Littlechild (2007) Acta Cryst. F63, 117-119.
Arabidopsis thaliana VTC4 encodes L-Galactose 1-P Phosphatase, a plant ascorbic acid biosynthetic enzyme
Patricia L. Conklin, Stephan Gatzek, Glen L. Wheeler, John Dowdle, Marjorie J. Raymond, Suzanne Rolinski, Mikhail Isupov, Jennifer Littlechild & Nicholas Smirnoff (2006) J. Biol. Chem. 281, 15662-15670
Anion binding tripodal receptors as structural models for the active site of vanadium haloperoxidases and acid phosphatases
Sophie Tapper, Jennifer Littlechild, Yann Molard, Ivan Prokes & James Tucker (2005) J. Supramolecular Chemistry, in press.
Synthesis and characterisation of a ligand that forms a stable tetrahedral intermediate in the active site of the Aureobacterium species (-) gamma-lactamase.
Stephen Connelly, Kirsty Line, Michail N Isupov & Jennifer A Littlechild. (2005). Org. Biomol. Chem., 3, 3260-3262.
Enhancing Effect of Calcium and Vanadium Ions on Thermal Stability of Bromoperoxidase from Corallina pilulifera
Takashi Ohshiro , Jennifer Littlechild, Esther Garcia-Rodriguez, Yasuaki Iida, Takushi Kobayashi & Yoshikazu Izumi (2005) J. Biological Inorganic Chemistry 10, 275-282.
The crystal structure of a (-) gamma-lactamase from an Aureobacterium species reveals a tetrahedral intermediate in the active site
K. Line, M. Isupov & J.A. Littlechild (2004) J.Mol. Biol. 338,519-532
Modification of halogen specificity of a vanadium dependent bromoperoxidase
Takashi Ohshiro , Jennifer Littlechild , Esther Garcia-Rodriguez, Michail N. Isupov, Yasuaki Iida, Takushi Kobayashi & Yoshikazu Izumi (2004) Protein Science 13, 1566-1571
Crystallisation and preliminary X-ray diffraction studies of a fungal hydrolase from Ophistoma novo-ulm
Michail N. Isupov, Amanda A. Brindley, Edward J. Hollingsworth, Garib N. Murshudov, Alexei A. Vagin & Jennifer A. Littlechild (2004) Acta Cryst.D,60, 1879-1882
Hyperthermophilic dehydrogenase Enzymes
J.A.Littlechild, J.E Guy & M.Isupov (2004) Biochemical Society Trans. 32, 255-258
Structural studies on the dodecameric vanadium bromoperoxidase from Corallina species.
Jennifer Littlechild & Esther Garcia-Rodriguez , (2003) Coordination Chemistry Reviews237,65-76.
Metal and Redox Regulation of Cysteine Protein Function
Niroshini M. Giles, Aaron Watts, Gregory I. Giles, Fiona H. Fry, Jennifer A. Littlechild & Claus Jacob . (2003) Chemistry and Biology 10,677-693.
The Use of a Thermostable Signature Amidase in the Resolution of the Bicyclic Synthon (rac)-γ-Lactam
Helen Toogood, Rob C. Brown, Kirsty Line, Phil Keene, Stephen Taylor, Ray McCague, & Jennifer Littlechild (2004) Tetrahedron 60, 711-716
The Structure of an Alcohol Dehydrogenase from the Hyperthermophilic Archaeon Aeropyrum pernix
Jodie E. Guy, Michail N. Isupov and Jennifer A. Littlechild.(2003) J.Mol.Biol. 331, 1041-1051.
Crystallisation and preliminary X-ray diffraction studies of a novel alcohol dehydrogenase from Aeropyrum pernix.
Jodie Guy, Michail Isupov & Jennifer Littlechild (2003) Acta Cryst. D, 59,174-176.
Structural and functional comparisons between vanadium haloperoxidase and acid phosphatase enzymes. Jennifer Littlechild , Esther Garcia, Andrew Dalby & Misha Isupov (2002) J.Mol.Recog. 15,291-296
A Thermostable L-Aminoacylase from Thermococcus litoralis: Cloning, Over-Expression, Characterisation and Applications in Biotransformations
Helen S. Toogood, Edward J. Hollingsworth, Rob C. Brown, Ian N. Taylor, Stephen J.C. Taylor, Ray McCague & Jennifer A. Littlechild (2002) Extremophiles 6, 111-122.
A Method for Screening Baeyer-Villiger Monooxygenase Activity Against Monocyclic Ketones
A.B.Watts, J. Beecher, C.S. Whitcher & J. A. Littlechild. (2002) Biocat. and Biotrans. 20, 209-214.
Immobilization of the Thermostable L-Aminoacylase from Thermococcus litoralis to Generate a Reusable Industrial Biocatalyst
Helen S. Toogood, Ian N. Taylor, Rob C. Brown, Stephen J.C. Taylor Ray McCague & Jennifer A. Littlechild (2002) Biocat. and Biotrans. 20, 241-249.
Crystallisation and Preliminary X-ray diffraction of L-aminoacylase from the hyperthermophilic archaeon Thermococcus litoralis.
Edward J. Hollingsworth, Michail N. Isupov & Jennifer Littlechild (2002) Acta Cryst. D58, 507-510.
Enantioselectivity of recombinant Rhizomucor miehei lipase in the ring opening of oxazolin-5(4H)-ones
N.A.Turner, D.J.H. Gaskin, A.T. Yagnik, J. A. Littlechild & E.N.Vulfson (2001) Protein Engng. 14, 269-278.
Chloroplast glyceraldehydes-3-phosphate dehydrogenase contains a single disulfide bond located in the C-terminal extension to the B subunit.
J.F. Qi, M.N. Isupov, J.A.Littlechild & L.E.Anderson (2001) J.Biol.Chem., 276, 35247-35252
The crystal structure of Human Liver Fructose 1,6 bisphoshate aldolase
A. R., Dalby, D. R. Tolan & J. A.Littlechild, (2001) Acta Cryst.D57 , 1526-1533.
Crystallisation and Preliminary X-Ray analysis of a gamma lactamase enzyme
I. Gonsalvez, M. N. Isupov & J. Littlechild (2001) Acta Cryst. D57, 284-286.
Comparison of the decameric structure of peroxiredoxin-II by transmission electron microscopy and X-ray crystallography.
J.R. Harris, E. Schroder, M. Isupov, D. Scheffler, P. Kristensen , J.A. Littlechild, A.A.Vagin & U. Meissner.(2001) Biochim et Biophys. Acta. 36419, 1-14.
Glyceraldehyde dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus.
Jennifer A. Littlechild & Michail Isupov (2001) Methods in Enzymology 331, Part B,105-117.
Phosphoglycerate Kinases from bacteria and archaea.
Gina Crowhurst, Jane McHarg & Jennifer Littlechild (2001) Methods in Enzymology 331, Part B 90-104.
Crystal structure of decameric 2-Cys Peroxiredoxin from himan erythrocytes at 1.7Å resolution.
Schroder, E. Littlechild, J.A. Lebedev, A.A. Errington, N. Vagin, A.A. & Isupov, M.N. (2000) Structure 8, 605-615.
Crystal Structure of Vanadium Dependent Bromoperoxidase from Corallina officinalis
Michail Isupov, Andrew Dalby, Amanda Brindley, Yoshikazu Izumi, Tadashi Tanabe & Jennifer Littlechild. (2000) J. Mol.Biol. 299, 1035-1049
Crystallisation and preliminary X-ray Diffraction Studies of Pyrrolidone-carboxyl peptidase from the hyperthermophilic Archaeon Thermococcus litoralis.
M.R. Singleton, M. N.Isupov & J.A. Littlechild. (1999) Acta Cryst. D55, 702-703.
Structure of Pyrrolidone-carboxyl peptidase from the hyperthermophilic Archaeon Thermococcus litoralis at 1.7A resolution.
M.R. Singleton, M. N.Isupov & J.A. Littlechild (1999) Structure 7, 237-244.
Crystallisation and Preliminary X-Ray Analysis of Human Natural Killer Enhancing Factor B from Red Blood Cells.
E. Schroder, M.N. Isupov, A. Naran & J. Littlechild, (1999) Acta Cryst. D55, 536-538.
Crystal Structure of Human Aldolase Complexed with Fructose 1,6 bisphosphate : Mechanistic Implications.
Dalby, Z. Dauter & J. Littlechild, (1999) Protein Science 8, 291-297.
Crystallisation and preliminary X-ray diffraction studies of a novel bacterial esterase
P.C. Bourne, M.N. Isupov & J.A. Littlechild. (1999) Acta Cryst. D55, 915-917.
Site directed mutagenesis of proline 204 in the hinge region of yeast phosphoglycerate kinase.
J.McHarg, S. M. Kelly, N. C. Price and A. Cooper & J. Littlechild. (1999) Eur. J. Biochem. 259, 939-945.
Crystal structure of the Glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus.
M. Isupov, T. Fleming, A. Dalby, G. S. Crowhurst, P.C. Bourne & J. Littlechild.(1999) J. Mol. Biol.291, 651-660.
X-ray crystallographic structure of a Phosphoglycerate Mutase - 3-phosphoglyceric acid complex at 1.7A resolution.
G. Crowhurst, A. Dalby, M. Isupov, J. Campbell & J. Littlechild (1999) Acta Cryst. D55, 1822-1826.
Crystallisation and preliminary X-ray diffraction studies of D-glyceraldehyde 3 phosphate dehydrogenase from the hyperthermophilic archaeon Methanothermus fervidus.
C. Charron, F. Talfournier, M.N. Isupov, G. Branlant, J.A. Littlechild, B. Vitoux & A. Aubry. (1999) Acta Cryst. D55, 1353-1355.
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